Effects of microglias and their cytokines on breast cancer cells

Yan LI, Ying BAI, Hai-ge WU, Ling XU

Abstract


Objective: To investigate the effect of conditioned medium (CM) of microglia N9 cells cultured in vitro on the proliferation of breast cancer MCF-7 cells, and to explore the possible mechanism of microglias in the brain metastasis. Methods: The N9 cells and MCF-7 cells were co-cultured for 12 h, and then the morphological changes of the two cells were observed by an inverted optical microscope. The N9 cells were stimulated by lipopolysaccharide (LPS), co-culture with MCF-7 cells and the conditioned medium (MCF-7-CM) to produce N9-CM. The tumor necrosis factor-α (TNF-α) and interleukin-17 (IL-17) in N9-CM were examined by ELISA, and the production of nitric oxide (NO) was detected by Griess system. MCF-7 cells were cultured with different concentrations (10%, 25%, 50%, 75% and 100%) of N9-CM. The survival rate of MCF-7 cells was detected by MTT method. Results: N9 cells were activated by co-culture with MCF-7 cells; and the secretions of NO, IL-17 and TNF-α were induced by LPS, MCF-7 cells and MCF-7-CM. The proliferative rates of MCF-7 cells treated with N9-CM at concentrations of 50%-100% were decreased in a time- and concentration-dependent manner. Conclusion: Microglias can be activated by metastatic breast cancer cells in central nerve system and decrease the proliferation of the breast cancer cells. This effect is positively related to the levels of NO, IL-17 and TNF-α secreted by microglias and the treatment time of these cytokines.

DOI:10.3781/j.issn.1000-7431.2012.05.004


Keywords


Breast neoplasms; Microglia; Neoplasm metastasis; Nitric oxide; Tumor necrosis factor-α; Interleukin-17

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